3W8G
MamM V260R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 61 |
| Unit cell lengths | 68.481, 68.481, 56.395 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.430 - 2.050 |
| R-factor | 0.1875 |
| Rwork | 0.184 |
| R-free | 0.25962 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5x |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.681 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 5.550 | 2.050 |
| Rmerge | 0.144 | 0.079 | 0.513 |
| Number of reflections | 8884 | ||
| <I/σ(I)> | 8.2 | ||
| Completeness [%] | 93.3 | 99.8 | 99.8 |
| Redundancy | 6.3 | 6.2 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M TRIS pH 7, 0.2M MgCl, 15% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
