3W7Z
1.15A structure of human 2Zn insulin at 293K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6C |
| Synchrotron site | Photon Factory |
| Beamline | BL-6C |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-04-13 |
| Detector | Fully automatic high speed Weissenberg data collection system called Galaxy |
| Wavelength(s) | 1.00 |
| Spacegroup name | H 3 |
| Unit cell lengths | 82.700, 82.700, 34.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.150 |
| R-factor | 0.14767 |
| Rwork | 0.145 |
| R-free | 0.19470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w7y |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.057 |
| Data reduction software | WEIS |
| Data scaling software | WEIS |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 41.500 |
| High resolution limit [Å] | 1.000 |
| Rmerge | 0.066 |
| Number of reflections | 46687 |
| Redundancy | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.67 | 293 | 10mg/ml protein, 0.1M Sodium Citrate, 22%(v/v) DMF, 0.08%(w/v) Zinc chloride, pH 8.67, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
