3W7Y

0.92A structure of 2Zn human insulin at 100K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6C
Synchrotron site	Photon Factory
Beamline	BL-6C
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-12-23
Detector	Fully automatic high speed Weissenberg data collection system called Galaxy
Wavelength(s)	0.97974
Spacegroup name	H 3
Unit cell lengths	81.120, 81.120, 33.930
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 0.920
R-factor	0.16196
Rwork	0.161
R-free	0.17951
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.187
Data reduction software	WEIS
Data scaling software	WEIS
Phasing software	CCP4
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	0.700
Rmerge	0.056
Number of reflections	57006
Completeness [%]	91.7
Redundancy	4.18

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.67	293	10mg/ml protein, 0.1M Sodium Citrate, 22% DMF(v/v) and 0.08% Zinc chloride(w/v), pH 8.67, VAPOR DIFFUSION, HANGING DROP, temperature 293K