3W7X
Crystal structure of E. coli YgjK D324N complexed with melibiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-05 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.140, 138.160, 86.200 |
| Unit cell angles | 90.00, 98.37, 90.00 |
Refinement procedure
| Resolution | 40.520 - 2.700 |
| R-factor | 0.19173 |
| Rwork | 0.189 |
| R-free | 0.24899 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3C68 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.131 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.116 | 0.343 |
| Number of reflections | 38773 | |
| <I/σ(I)> | 4.9 | |
| Completeness [%] | 98.0 | 97.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 20% PEG 8000, 0.6M magnesium chloride, 100mM Tris-HCl buffer, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
