3W6Z
Crystal structure of NADP bound L-serine 3-dehydrogenase (K170M) from Hyperthermophilic Archaeon Pyrobaculum calidifontis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-30 |
| Detector | PILATUS 2M-F |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.996, 57.348, 56.393 |
| Unit cell angles | 90.00, 106.73, 90.00 |
Refinement procedure
| Resolution | 46.800 - 1.440 |
| Rwork | 0.172 |
| R-free | 0.18500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w6u |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 57.938 |
| High resolution limit [Å] | 1.440 |
| Number of reflections | 67330 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 2.0M AMMONIUM SULFATE, 0.1M ACETATE BUFFER, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
