3W6R
Crystal structure of the GAP domain of human MgcRacGAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-03 |
| Wavelength(s) | 0.97928 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.466, 63.159, 74.078 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.310 - 1.900 |
| R-factor | 0.22346 |
| Rwork | 0.222 |
| R-free | 0.25873 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ovj |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.946 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.085 | |
| Number of reflections | 15787 | |
| <I/σ(I)> | 51.6 | 5.2 |
| Completeness [%] | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | 295 | 100mM sodium cacodylate buffer, 13% polyethylene glycol 6000, 25% glycerol, pH 5.7, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
