3W6Q
Crystal structure of melB apo-protyrosinase from Asperugillus oryzae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-07 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.968, 108.752, 231.235 |
Unit cell angles | 90.00, 93.83, 90.00 |
Refinement procedure
Resolution | 39.107 - 2.052 |
R-factor | 0.1458 |
Rwork | 0.144 |
R-free | 0.18290 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.004 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX ((AutoSol)) |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 175066 | |
Completeness [%] | 97.7 | 84.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 20% PEG 3350, 50mM potassium Chloride, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |