3W6K
Crystal structure of dimer of ScpB N-terminal domain complexed with ScpA peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.823, 88.919, 60.683 |
| Unit cell angles | 90.00, 94.31, 90.00 |
Refinement procedure
| Resolution | 46.476 - 2.374 |
| R-factor | 0.2383 |
| Rwork | 0.236 |
| R-free | 0.28460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Dimer of Geobacillus stearothermophilus ScpB N-terminal domain |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.296 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.420 |
| High resolution limit [Å] | 2.374 | 2.374 |
| Rmerge | 0.051 | 0.116 |
| Number of reflections | 23087 | |
| <I/σ(I)> | 37.406 | 11.464 |
| Completeness [%] | 99.4 | 89.8 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 100mM Na malonate-HCl, 3.0-3.3M Na formate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
