3W6J
Crystal structure of ScpAB core complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.856, 127.423, 97.218 |
| Unit cell angles | 90.00, 113.84, 90.00 |
Refinement procedure
| Resolution | 30.700 - 2.600 |
| R-factor | 0.2267 |
| Rwork | 0.225 |
| R-free | 0.26410 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.181 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.063 | 0.669 |
| Number of reflections | 61981 | |
| <I/σ(I)> | 19.793 | 1.625 |
| Completeness [%] | 98.5 | 89.5 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 0.49M NaH2PO4, 0.91M K2HPO4, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
