3W66
MamM-CTD D249A and H285A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-12-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5417 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 34.685, 93.866, 50.951 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.480 - 2.050 |
| R-factor | 0.1846 |
| Rwork | 0.182 |
| R-free | 0.24240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5x |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.903 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 5.550 | 2.050 |
| Rmerge | 0.059 | 0.028 | 0.324 |
| Number of reflections | 5384 | ||
| <I/σ(I)> | 11.1 | ||
| Completeness [%] | 97.9 | 100 | 84.3 |
| Redundancy | 7.7 | 7.4 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1M BIS-TRIS PH 5.5, 25% PEG 3350, 0.2M AmSO4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
