3W64
MamM-CTD 215-293
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-05-12 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.966, 75.477, 88.405 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.890 - 2.850 |
| R-factor | 0.2252 |
| Rwork | 0.223 |
| R-free | 0.26960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5x |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.695 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.900 |
| High resolution limit [Å] | 2.850 | 7.600 | 2.850 |
| Rmerge | 0.126 | 0.053 | 0.494 |
| Number of reflections | 10553 | ||
| <I/σ(I)> | 7.4 | ||
| Completeness [%] | 99.7 | 99.7 | 99.8 |
| Redundancy | 6.7 | 6.5 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 293 | 0.1M BIS-TRIS PH 6.9, 25% PEG 3350, 0.2M LiSO4 , VAPOR DIFFUSION, SITTING DROP, temperature 293K |
