3W5W
Mn2+-GMP complex of nanoRNase (Nrn) from Bacteroides fragilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 90.828, 90.828, 107.878 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.950 |
| Rwork | 0.215 |
| R-free | 0.27010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3DMA |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.313 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
| High resolution limit [Å] | 2.950 | 8.000 | 2.950 |
| Rmerge | 0.092 | 0.032 | 0.302 |
| Number of reflections | 11209 | ||
| <I/σ(I)> | 9.1 | ||
| Completeness [%] | 99.8 | 96.8 | 100 |
| Redundancy | 21.9 | 19.6 | 22.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | 0.1M sodium acetate trihydrate (pH 5.2), 1.6M NaCl, 5mM MgCl2, 10mM 3'-phosphoguanosine 5' phophate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
