3W5U
Cross-linked complex between Ferredoxin and Ferredoxin-NADP+ reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-18 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.202, 153.634, 100.107 |
| Unit cell angles | 90.00, 99.42, 90.00 |
Refinement procedure
| Resolution | 45.460 - 2.700 |
| R-factor | 0.22902 |
| Rwork | 0.226 |
| R-free | 0.27975 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gaw 3b2f |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.318 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 150.000 |
| High resolution limit [Å] | 2.680 |
| Number of reflections | 48206 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20% PEG 6000, 50mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
