3W5M
Crystal Structure of Streptomyces avermitilis alpha-L-rhamnosidase
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2008-06-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97909 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.769, 128.414, 75.173 |
| Unit cell angles | 90.00, 99.34, 90.00 |
Refinement procedure
| Resolution | 35.600 - 1.800 |
| R-factor | 0.18133 |
| Rwork | 0.180 |
| R-free | 0.21401 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.397 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.660 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 92680 | |
| <I/σ(I)> | 36.9 | 4.3 |
| Completeness [%] | 99.8 | 98.5 |
| Redundancy | 6.9 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 293 | 10% (w/v) PEG 6000, 5% (v/v) MPD, 0.1M HEPES, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
