3W57
Structure of a C2 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-10-04 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.803, 52.751, 49.055 |
| Unit cell angles | 90.00, 107.15, 90.00 |
Refinement procedure
| Resolution | 20.014 - 1.662 |
| R-factor | 0.1605 |
| Rwork | 0.159 |
| R-free | 0.18190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nsj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.117 |
| Data scaling software | SCALA (3.3.16) |
| Refinement software | PHENIX (dev_818) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.873 | 20.014 | 1.750 |
| High resolution limit [Å] | 1.662 | 5.260 | 1.660 |
| Rmerge | 0.028 | 0.267 | |
| Rmeas | 0.032 | 0.310 | |
| Rpim | 0.015 | 0.153 | |
| Total number of observations | 3592 | 13062 | |
| Number of reflections | 24949 | ||
| <I/σ(I)> | 19.8 | 39.3 | 5.2 |
| Completeness [%] | 98.6 | 98.2 | 90.9 |
| Redundancy | 4.3 | 4.3 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 100mM Tris-HCl, 200mM CaCl2, 20% PEG 3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
