3W53
Crystal structure of psychrophilic beta-glucosidase BglU from Micrococcus antarcticus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-09-27 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 57.280, 84.670, 184.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.443 - 2.200 |
R-factor | 0.1632 |
Rwork | 0.161 |
R-free | 0.19970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2z1s |
RMSD bond length | 0.007 |
RMSD bond angle | 1.070 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.550 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.039 | 0.073 |
Number of reflections | 22807 | |
<I/σ(I)> | 32.6 | 14.2 |
Completeness [%] | 97.9 | 87.4 |
Redundancy | 6.4 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.2(mol/L) MgCl2, 30% PEG4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |