3W52
Zinc-dependent bifunctional nuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9732 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.254, 66.727, 99.487 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.760 |
| R-factor | 0.171 |
| Rwork | 0.167 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ak0 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.820 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.084 | 0.337 |
| Number of reflections | 36392 | |
| <I/σ(I)> | 27.4 | 4.6 |
| Completeness [%] | 99.4 | 94 |
| Redundancy | 7.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 50mM NaCl, 20mM Tris(pH7.5), 125 micro-M thiophosphorylated adenosine hexanucleotide, 30%(w/v) PEG3350, 0.2M Li2SO4, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
