3W4Y
Crystal structure of yeast Erv1 core
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-26 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 62.531, 116.222, 151.119 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.870 - 2.000 |
| R-factor | 0.20698 |
| Rwork | 0.206 |
| R-free | 0.23077 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hj3 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.324 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.870 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 37584 |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 10-20 % Polyethyleneglycole, 0.2-0.4 M citrate-Na, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
