3W4U
Human zeta-2 beta-2-s hemoglobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-06-18 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 115.540, 115.540, 140.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.950 - 1.950 |
| R-factor | 0.2076 |
| Rwork | 0.205 |
| R-free | 0.25470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jeb |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.778 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.950 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.079 | 0.415 |
| Number of reflections | 79112 | |
| <I/σ(I)> | 17.6 | 2.4 |
| Completeness [%] | 99.5 | 98.7 |
| Redundancy | 13.4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 298 | 3.1M sodium potassium phosphate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
