3W4T
Crystal structure of MATE P26A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-11 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 155.388, 60.055, 68.279 |
| Unit cell angles | 90.00, 92.00, 90.00 |
Refinement procedure
| Resolution | 31.645 - 2.096 |
| R-factor | 0.1904 |
| Rwork | 0.189 |
| R-free | 0.21690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.829 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.5.0) |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
| High resolution limit [Å] | 2.096 | 5.700 | 2.100 |
| Rmerge | 0.104 | 0.051 | 0.368 |
| Number of reflections | 36428 | ||
| <I/σ(I)> | 7.6 | ||
| Completeness [%] | 97.7 | 99.4 | 98.4 |
| Redundancy | 5.5 | 6.7 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | lipdic cubic phase | 6.5 | 293 | 30% PEG 400, 100mM MES-NaOH, 100mM NaSCN, 20mM CaCl2, pH 6.5, lipdic cubic phase, temperature 293K |
