3W45
Crystal structure of RsbX in complex with cobalt in space group P1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.6047 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.108, 41.614, 68.806 |
| Unit cell angles | 80.81, 89.95, 71.08 |
Refinement procedure
| Resolution | 19.670 - 1.700 |
| R-factor | 0.1716 |
| Rwork | 0.170 |
| R-free | 0.20230 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.516 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 34187 | |
| <I/σ(I)> | 12.7 | 2.8 |
| Completeness [%] | 91.2 | 60.4 |
| Redundancy | 7.9 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | 20% PEG 1000, 0.1M Tris-HCl, 3mM CoCl2, pH 8.5, VAPOR DIFFUSION, temperature 298K |
