3W44
Crystal structure of RsbX, selenomethionine derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-06 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9780 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 85.610, 85.610, 103.983 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.19052 |
| Rwork | 0.188 |
| R-free | 0.24036 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.903 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE (2.13) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 19928 | |
| <I/σ(I)> | 13.64 | 2.57 |
| Completeness [%] | 98.2 | 87.4 |
| Redundancy | 3.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | 27% PEG 4000, 90mM Tris-HCl, 0.18M MgCl2, 0.12M MnCl2, pH 8.5, VAPOR DIFFUSION, temperature 298K |
