3W41
Crystal structure of RsbX in complex with magnesium in space group P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.176, 68.248, 38.714 |
| Unit cell angles | 90.00, 108.50, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.420 |
| R-factor | 0.1887 |
| Rwork | 0.187 |
| R-free | 0.21390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w45 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.418 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.470 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Number of reflections | 30818 | |
| <I/σ(I)> | 27.9 | 3.4 |
| Completeness [%] | 98.3 | 99.9 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | 20% PEG 1000, 100mM Tris-HCl, 5mM MgCl2, pH 8.5, VAPOR DIFFUSION, temperature 298K |
