3W37
Sugar beet alpha-glucosidase with acarbose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-06 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.360, 98.200, 108.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.527 - 1.700 |
| R-factor | 0.1541 |
| Rwork | 0.154 |
| R-free | 0.18170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.228 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.2_860) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.180 | 1.790 | |
| High resolution limit [Å] | 1.700 | 5.340 | 1.700 |
| Rmerge | 0.103 | 0.046 | 0.880 |
| Rmeas | 0.051 | 0.971 | |
| Number of reflections | 102030 | 3504 | 14446 |
| <I/σ(I)> | 11.84 | 28.08 | 2.97 |
| Completeness [%] | 99.9 | 98.8 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 50mM ammonium sulfate, 50mM sodium acetate buffer, 16% PEG monomethylether 2000, 14mM acarbose, pH 4.0, vapor diffusion, hanging drop, temperature 298K |
