3W31
Structual basis for the recognition of Ubc13 by the Shigella flexneri effector OspI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Collection date | 2011-12-14 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 119.238, 119.238, 69.985 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.960 |
| Rmerge | 0.094 |
| Number of reflections | 12193 |
| Completeness [%] | 99.8 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.4M potassium iodide, 0.1M MES (pH6.5), 4mM DTT, 15% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
