3W1Z
Heat shock protein 16.0 from Schizosaccharomyces pombe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 78.296, 121.078, 122.592 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.834 - 2.401 |
| R-factor | 0.2478 |
| Rwork | 0.246 |
| R-free | 0.28640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gme |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.049 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 22653 | |
| <I/σ(I)> | 21.4 | 2.1 |
| Completeness [%] | 97.8 | 95.6 |
| Redundancy | 5.3 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 15% PEGMME550, 100mM sodium chloride, 100mM Bicine buffer, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
