3W1D
Structure of a pressure sensitive YFP variant YFP-G3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.050, 62.980, 70.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.280 - 1.500 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dq7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.300 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.066 | 0.415 |
| Number of reflections | 36353 | |
| <I/σ(I)> | 17.5 | 3.4 |
| Completeness [%] | 98.6 | 91.6 |
| Redundancy | 6.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 13-17% PEG 8000, 0.1M Bis-Tris buffer, 200-400mM calcium acetate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
