3W1C
Structure of a pressure sensitive YFP variant YFP-G1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.190, 62.760, 69.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.250 - 1.300 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dq7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.300 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.065 | 0.469 |
| Number of reflections | 54434 | |
| <I/σ(I)> | 15.9 | 3.5 |
| Completeness [%] | 97.3 | 94.5 |
| Redundancy | 5.6 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 13-15% PEG 8000, 0.1M Bis-Tris buffer, 350-400mM calcium acetate , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
