3W03
XLF-XRCC4 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 65 |
| Unit cell lengths | 236.762, 236.762, 103.229 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.810 - 8.492 |
| R-factor | 0.3622 |
| Rwork | 0.363 |
| R-free | 0.35990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qm4 1fu1 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX ((1.7.2_869)) |
| Refinement software | PHENIX ((1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.810 | 8.800 |
| High resolution limit [Å] | 8.492 | 8.500 |
| Rmerge | 0.050 | 0.445 |
| Number of reflections | 2995 | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.4 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M Tris pH7.5, 2M Sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
