3VZZ
Crystal structure of PcrB complexed with FsPP from bacillus subtilis subap. subtilis str. 168
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.136, 56.078, 77.523 |
| Unit cell angles | 90.00, 108.04, 90.00 |
Refinement procedure
| Resolution | 25.060 - 2.040 |
| Rwork | 0.178 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vzx |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.060 | 2.110 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.059 | 0.319 |
| Number of reflections | 27153 | |
| <I/σ(I)> | 21.7 | 3.4 |
| Completeness [%] | 96.4 | 91.7 |
| Redundancy | 4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 2.5M NaCl, 14% PEG6000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
