3VZW
Crystal Structure of outer membrane protein PorB from Neisseria meningitidis in complex with galactose
Replaces: 3A2TExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-31 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 63 |
| Unit cell lengths | 84.343, 84.343, 105.952 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.180 - 3.200 |
| R-factor | 0.22743 |
| Rwork | 0.226 |
| R-free | 0.25087 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3A2R |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.627 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.280 |
| High resolution limit [Å] | 3.200 | |
| Number of reflections | 6570 | |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 91.7 | 51.5 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 100mM MES, 50mM CsCl, 28-32% Jeffamine M-600, pH6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
