3VZM
Crystal structure of the Bacillus circulans endo-beta-(1,4)-xylanase (BcX) E172H mutant with Glu78 covalently bonded to 2-deoxy-2-fluoro-xylobiose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-07-01 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.541 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.451, 52.387, 77.768 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.430 - 1.860 |
R-factor | 0.17517 |
Rwork | 0.172 |
R-free | 0.25372 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hv1 |
RMSD bond length | 0.022 |
RMSD bond angle | 1.848 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 60.000 |
High resolution limit [Å] | 1.670 |
Number of reflections | 49715 |
Completeness [%] | 92.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 13-20% (NH4)2SO4, 40mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |