3VZK
Crystal structure of the Bacillus circulans endo-beta-(1,4)-xylanase (BcX) N35E mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-06-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.541 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.327, 62.814, 64.126 |
| Unit cell angles | 90.00, 104.57, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.550 |
| R-factor | 0.16343 |
| Rwork | 0.162 |
| R-free | 0.19672 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hv1 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.944 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 62.000 |
| High resolution limit [Å] | 1.540 |
| Number of reflections | 62680 |
| Completeness [%] | 97.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 13-20% (NH4)2SO4, 40mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
