3VZJ
Crystal structure of the Bacillus circulans endo-beta-(1,4)-xylanase (BcX) E172H mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-06-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.541 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.174, 86.737, 73.090 |
| Unit cell angles | 90.00, 89.89, 90.00 |
Refinement procedure
| Resolution | 38.167 - 2.406 |
| R-factor | 0.2442 |
| Rwork | 0.239 |
| R-free | 0.34340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hv1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.237 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 38.167 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 31471 |
| Completeness [%] | 91.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 13-20% (NH4)2SO4, 40mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
