3VZ8

Crystal Structure Analysis of the Mini-chaperonin variant with Leu 185, Val 186, Pro 187, Arg 188 and Ser 190 replaced with all Gly

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2001-02-27
Detector	ADSC QUANTUM 4r
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	95.058, 38.010, 102.204
Unit cell angles	90.00, 116.23, 90.00

Refinement procedure

Resolution	47.510 - 1.900
R-factor	0.263
Rwork	0.263
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kid
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	EPMR
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.020
High resolution limit [Å]	1.900	1.900
Number of reflections	52185
Completeness [%]	99.4	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	288	0.1M Tris-HCl, 1.61M NaCl, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 288K