3VZ7

Crystal Structure Analysis of the mini-chaperonin variant with Pro 187 Gly

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2000-08-10
Detector	ADSC QUANTUM 4r
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	75.990, 84.810, 35.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.930 - 1.800
R-factor	0.227
Rwork	0.227
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kid
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	EPMR
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.910
High resolution limit [Å]	1.800	1.800
Number of reflections	21847
Completeness [%]	99.7	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.7	288	0.1M Tris-HCl, 1M NaCl, 3.75% Glycerol, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 288K