3VYX
Structural insights into RISC assembly facilitated by dsRNA binding domains of human RNA helicase (DHX9)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 63.198, 63.198, 107.617 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.270 - 2.290 |
| R-factor | 0.19639 |
| Rwork | 0.193 |
| R-free | 0.26312 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uil |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.715 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.290 | 2.300 |
| Number of reflections | 9800 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.8 | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG 4000, KNO3, Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
