3VYS

Crystal structure of the HypC-HypD-HypE complex (form I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	78.567, 99.201, 104.025
Unit cell angles	90.00, 97.69, 90.00

Refinement procedure

Resolution	38.930 - 2.350
R-factor	0.218
Rwork	0.218
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z1c 2z1d 2z1e
RMSD bond length	0.011
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.390
High resolution limit [Å]	2.350	2.350
Number of reflections	32417
<I/σ(I)>	20.9	2
Completeness [%]	98.0	94.3
Redundancy	3.3	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	293	50mM MES, 12-16% PEG400, 10mM magnesium chloride, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K