3VYO
Crystal structure of Mycobacterium tuberculosis L,D-transpeptidase LdtMt2 N140 truncation mutant (resideus 140-408)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 98 |
| Detector technology | CCD |
| Collection date | 2012-06-13 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97889 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 116.370, 121.190, 122.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.270 - 1.800 |
| R-factor | 0.2094 |
| Rwork | 0.209 |
| R-free | 0.22490 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.307 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.190 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.112 | 0.564 |
| Number of reflections | 80508 | |
| <I/σ(I)> | 9.8 | 2.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.5 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.2M magnesium acetate, 0.1M sodium cacodylate, 30% PEG8000 , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
