3VYN
Crystal structure of Mycobacterium tuberculosis L,D-transpeptidase LdtMt2 N55 truncation mutant (resideus 55-408)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 98 |
| Detector technology | CCD |
| Collection date | 2012-06-13 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.96422 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.100, 93.150, 75.500 |
| Unit cell angles | 90.00, 92.86, 90.00 |
Refinement procedure
| Resolution | 48.640 - 2.500 |
| R-factor | 0.2198 |
| Rwork | 0.220 |
| R-free | 0.26140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vyo |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.030 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.149 | 0.497 |
| Number of reflections | 29308 | |
| <I/σ(I)> | 6.8 | 2.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 100mM Bis-Tis, 200mM MgCl2, 20% PEG3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
