3VY9
Crystal structure of PorB from Neisseria meningitidis in complex with cesium ion, space group H32
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.918 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 144.208, 144.208, 106.411 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.630 |
| R-factor | 0.23988 |
| Rwork | 0.235 |
| R-free | 0.28067 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3A2R |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.908 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.000 | 2.700 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Number of reflections | 11968 | |
| Completeness [%] | 94.3 | 76.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 30% Jeffamine M600, 50mM CsCl, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
