3VXB
Crystal Structure of BxlE from Streptomyces thermoviolaceus OPC-520
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS VII |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.634, 63.272, 66.402 |
| Unit cell angles | 90.00, 103.05, 90.00 |
Refinement procedure
| Resolution | 29.310 - 1.850 |
| R-factor | 0.19202 |
| Rwork | 0.190 |
| R-free | 0.23997 |
| Structure solution method | MAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.835 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 34.200 |
| High resolution limit [Å] | 1.650 |
| Rmerge | 0.026 |
| Number of reflections | 39659 |
| Completeness [%] | 91.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.2 | 293 | 25% PEG 3350, 0.1M citrate, pH 3.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.2 | 293 | 25% PEG 3350, 0.1M citrate, pH 3.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
