3VWV
crystal structure of N-terminally truncated peroxiredoxin 4 from M. musculus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-06 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | I 4 |
| Unit cell lengths | 104.321, 104.321, 84.228 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.810 - 1.800 |
| R-factor | 0.1552 |
| Rwork | 0.153 |
| R-free | 0.19320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.469 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.810 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 41798 | |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 7.6 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.6 | 293 | 30% PEG 400, 100mM NaOAc (pH 4.6), 100mM CaCl2, EVAPORATION, temperature 293K |
