3VWE
Crystal structure of alpha-tubulin acetyltransferase domain of human Mec-17 in complex with CoA (P21 form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-15 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.100 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.726, 34.998, 63.981 |
| Unit cell angles | 90.00, 103.24, 90.00 |
Refinement procedure
| Resolution | 39.621 - 1.956 |
| R-factor | 0.1819 |
| Rwork | 0.180 |
| R-free | 0.22520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.102 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 5.290 | 1.950 |
| Rmerge | 0.141 | 0.115 | 0.508 |
| Number of reflections | 13656 | ||
| <I/σ(I)> | 7.6 | ||
| Completeness [%] | 98.2 | 99.1 | 98.8 |
| Redundancy | 2.8 | 3.3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 0.1M Tris, 26% PEG 3350, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
