3VWB
Crystal structure of VirB core domain (Se-Met derivative) complexed with the cis-acting site (5-BRU modifications) upstream icsb promoter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-05 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 57.321, 163.051, 39.569 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.891 - 2.416 |
| R-factor | 0.2371 |
| Rwork | 0.235 |
| R-free | 0.27100 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.979 |
| Data reduction software | XDS (package) |
| Data scaling software | XDS (package) |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.891 | 2.560 |
| High resolution limit [Å] | 2.416 | 2.420 |
| Rmerge | 0.067 | 0.768 |
| Number of reflections | 27467 | |
| <I/σ(I)> | 21.56 | 2.43 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 7.77 | 7.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 295 | 0.1M MgCl2, 0.1M Sodium Acetate, 22% PEG400, 3%(v/v) dimethyl sulfoxide, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
