3VWA
Crystal structure of Cex1p
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-20 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97914, 0.97941, 0.96410 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.056, 182.222, 112.440 |
| Unit cell angles | 90.00, 98.69, 90.00 |
Refinement procedure
| Resolution | 45.560 - 2.200 |
| R-factor | 0.1698 |
| Rwork | 0.166 |
| R-free | 0.20680 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.821 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 5.970 | 2.200 |
| Rmerge | 0.099 | 0.047 | 0.630 |
| Number of reflections | 67587 | ||
| <I/σ(I)> | 8 | ||
| Completeness [%] | 98.9 | 100 | 88.5 |
| Redundancy | 14.5 | 15.3 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 7% PEG 3350, 100mM Na-tartrate, 3% Isopropanol, 50mM HEPES, pH 7.5, vapor diffusion, sitting drop, temperature 293K |
