3VW1
Crystal Strucuture of the Crystal Violet-Bound Form of RamR (Transcriptional Regurator of TetR Family) from Salmonella Typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Detector technology | CCD |
| Collection date | 2012-01-23 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.594, 54.741, 92.711 |
| Unit cell angles | 104.45, 97.25, 89.93 |
Refinement procedure
| Resolution | 22.280 - 2.210 |
| R-factor | 0.2214 |
| Rwork | 0.220 |
| R-free | 0.25360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.522 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 5.970 | 2.200 |
| Rmerge | 0.031 | 0.022 | 0.219 |
| Number of reflections | 40122 | ||
| <I/σ(I)> | 20.7 | ||
| Completeness [%] | 97.3 | 77.3 | 96.2 |
| Redundancy | 3.9 | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M Mes, 20mM NaPi, 75mM NaCl, 0.2M Ammonium sulfate, 2mM DTT, 20% PEG 6000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
