3VW0
Crystal Structure of The Dequalinum-bound Form of RamR (Transcriptional Regulator of TetR Family) From Salmonella Typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-23 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 187.345, 43.551, 106.692 |
| Unit cell angles | 90.00, 100.21, 90.00 |
Refinement procedure
| Resolution | 38.150 - 2.600 |
| R-factor | 0.2263 |
| Rwork | 0.224 |
| R-free | 0.26680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.570 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 7.050 | 2.600 |
| Rmerge | 0.072 | 0.029 | 0.458 |
| Number of reflections | 26448 | ||
| <I/σ(I)> | 11.7 | ||
| Completeness [%] | 99.8 | 96.4 | 100 |
| Redundancy | 7.4 | 6.5 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.67 | 298 | 0.1M MES, 20mM NaPi, 75mM NaCl, 0.2M Ammonium sulfate, 2mM DTT, 25% PEG 6000, pH 6.67, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
