3VVW

NDP52 in complex with LC3C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	195.720, 37.420, 40.280
Unit cell angles	90.00, 94.66, 90.00

Refinement procedure

Resolution	40.147 - 2.500
R-factor	0.2121
Rwork	0.210
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.046
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (dev_723)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.770	48.770	2.640
High resolution limit [Å]	2.500	7.910	2.500
Rmerge		0.060	0.446
Number of reflections	10306
<I/σ(I)>		17.6	3.2
Completeness [%]	99.8	98.8	100
Redundancy		3.6	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	16% PEG6000, 0.01M sodium citrate , pH 7.0, Vapor Diffusion, temperature 293K