3VVT
Crystal structure of reconstructed archaeal ancestral NDK, Arc1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 73 |
| Detector technology | CCD |
| Collection date | 2007-01-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 110.398, 110.398, 110.398 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.520 - 2.400 |
| R-factor | 0.187 |
| Rwork | 0.186 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cwk |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.986 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.071 | 0.539 |
| Number of reflections | 17715 | |
| <I/σ(I)> | 38.379 | |
| Completeness [%] | 99.8 | 98.1 |
| Redundancy | 10.2 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES, 0.8M POTTASIUM SODIUM TARTRATE TETRAHYDRATE, 3% 2-METHYL-2,4,-PENTADIOL, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
